Measurement of endogenous lysophosphatidic acid by ESI-MS/MS in plasma samples requires pre-separation of lysophosphatidylcholine

Published Date
1 November 2009, Vol.877(29):3739–3742, doi:10.1016/j.jchromb.2009.08.032
Short communication

Author 

• Zhenwen Zhao
 
• Yan Xu ^,

• Department of Obstetrics and Gynecology, Indiana University Cancer Center, Indiana University School of Medicine, USA

Received 6 March 2009. Accepted 23 August 2009. Available online 27 August 2009.

Abstract 

The levels of lysophosphatidic acid (LPA) or lysophosphatidylcholine (LPC) in plasma have been shown to be markers for several human diseases, including cancers. Here we show that the presence of LPC or other lysophospholipids (LPLs) in lipids extracted from biological samples affects accurate measurement of endogenous LPA in biological samples. We report for the first time the artificial conversion of LPC and lysophosphatidylserine (LPS) to LPA at the ion source of electrospray ionization tandem mass spectrometry (ESI-MS/MS). To avoid the interference of LPC with the quantification of LPA, a method based on high-performance liquid chromatography (HPLC) separation of LPA from LPC has been developed.

Keywords

High-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC–ESI-MS/MS)

Lysophosphatidic acid (LPA)

Lysophosphatidylcholine (LPC)

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  Corresponding author at: 975 W. Walnut St. IB355A, Indianapolis, IN 46202, USA. Tel.: +1 317 274 3972; fax: +1 317 278 4828.

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