Published Date
Energy Procedia
November 2015, Vol.79:784–790, doi:10.1016/j.egypro.2015.11.567
2015 International Conference on Alternative Energy in Developing Countries and Emerging Economies
Open Access, Creative Commons license
Oil palm frond
Preteatment
Simultaneous saccharification and fermentation
For further details log on website :
http://www.sciencedirect.com/science/article/pii/S1876610215022997
Energy Procedia
November 2015, Vol.79:784–790, doi:10.1016/j.egypro.2015.11.567
2015 International Conference on Alternative Energy in Developing Countries and Emerging Economies
Open Access, Creative Commons license
Available online 3 December 2015.
Abstract
Ethanol production from oil palm frond (OPF) by simultaneous saccharification and Saccharomyces cerevisiae TISTR5048 fermentation was investigated. Solid fraction of OPF (20% TS) was pretreated by 2% H2SO4, 2% NaOH and 2% NaOH in H2O2presoaking at room temperature for 24 hours. Pretreated OPF by presoaking in 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 contained 37%, 42% and 49% of cellulose, respectively. Pretreated OPF was simultaneous saccharification by cellulase enzyme (Cellic CTec2, Novozymes) and sequentially fermentation. Sugar concentration in OPF cellulose hydrolysis of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 45.72, 55.73 and 56.94 g/l, respectively. Ethanol concentration of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 14.5, 15.0 and 17.2 g/L, respectively. 2% NaOH in H2O2 presoaking was the best pretreatment with 82.11% of total solids recovery and containing 49.9% of cellulose with enzyme digestion ability of 37.6%.
Keywords
Ethanol production
Abstract
Ethanol production from oil palm frond (OPF) by simultaneous saccharification and Saccharomyces cerevisiae TISTR5048 fermentation was investigated. Solid fraction of OPF (20% TS) was pretreated by 2% H2SO4, 2% NaOH and 2% NaOH in H2O2presoaking at room temperature for 24 hours. Pretreated OPF by presoaking in 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 contained 37%, 42% and 49% of cellulose, respectively. Pretreated OPF was simultaneous saccharification by cellulase enzyme (Cellic CTec2, Novozymes) and sequentially fermentation. Sugar concentration in OPF cellulose hydrolysis of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 45.72, 55.73 and 56.94 g/l, respectively. Ethanol concentration of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 14.5, 15.0 and 17.2 g/L, respectively. 2% NaOH in H2O2 presoaking was the best pretreatment with 82.11% of total solids recovery and containing 49.9% of cellulose with enzyme digestion ability of 37.6%.
Keywords
References
- [1]
- Zhu.S.,Wu.Y.,Yu.Z.,Zhang. Wang.c.,Yu.f.,Jin.S. Production of ethanol form microwave-assisted alkali pretreatment wheat straw. Proc Biochem. 2006; 41, p. 869-873.
- [2]
- Sukumaran.R.K., Singhania. R. R., Mathew. G. M., Pandey. A. Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production. Renew. Energy 2009; 34, p. 421-424.
- [3]
- Prodroction of from pulp obtained by steam explosion pretreatment of oil palm trunk
- Energy sources, Volume 33, 2011, pp. 221–228
- [4]
- Kim, S. and Kim, C.H. Bioethanol production using the sequential acid/alkali- pretreated empty palm fruit bunch fiber. Renew. Energy. 2012; 3, p.1-6.
- [5]
- Boonsawang, P., Subkaree, Y. and Srinorakutara, T. Ethanol production from palm pressed fiber by prehydrolysis prior to simultaneous saccharification and fermentation (SSF). Biomass Bioenergy. 2012; 40, p.127-132.
- [6]
- Srimachai,T., Thonglimp, Thonglimp.V, and O-Thong, S. Ethanol and Methane Production from Oil Palm Frond by Two stage SSF. Energy Procedia 2014; 52, p. 352-361.
- [7]
- Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chemy 1959; 31, p. 420-8.
- [8]
- Dence, C.W. and Lin, S.Y. The determination of lignin,” In: Lin, S.Y., Dence, C.W. (Eds.). Methods in Lignin Chemistry. 1992; p. 33-61. Berlin: Springer-Verlag.
- [9]
- Sun, J.X., Sun, X.F., Zhao, H. and Sun, R.C. Isolation and characterization of cellulose from sugarcane bagasse. Polym. Degrad. Stab. 2004; 84, p. 331-339.
- ☆Peer-review under responsibility of the Organizing Committee of 2015 AEDCEE.
- ⁎ Corresponding author. Tel.: +66 746 09600; fax: +66 746 93992.
Copyright © 2015 The Authors. Published by Elsevier Ltd.
http://www.sciencedirect.com/science/article/pii/S1876610215022997
No comments:
Post a Comment