High-yield production of biosugars from Gracilaria verrucosa by acid and enzymatic hydrolysis processes

Published Date
Bioresource Technology
November 2015, Vol.196:634–641, doi:10.1016/j.biortech.2015.08.016

• Author 

• Se Won Kim ^a
• Chae-Hwan Hong ^b
• Sung-Wan Jeon ^b
• Hyun-Jae Shin ^a,,

• aDepartment of Chemical, Biochemical, Chosun University, Gwnagju, Republic of Korea
• bResearch and Development Division, Hyundai Motor Group, Uiwang, Republic of Korea

Received 5 June 2015. Revised 7 August 2015. Accepted 8 August 2015. Available online 14 August 2015. 

Highlights

• •
  Red algae was used for biosugars production by a combined hydrolysis approach.
• •
  Biosugar yield was 0.427 g/g for combined hydrolysis and conversion was 57.2%.
• •
  Mass balance of the experimental results from the combined processes was analyzed.

Abstract

Gracilaria verrucosa, the red alga, is a suitable feedstock for biosugar production. This study analyzes biosugar production by the hydrolysis of G. verrucosa conducted under various conditions (i.e., various acid concentrations, substrate concentrations, reaction times, and enzyme dosages). The acid hydrolysates of G. verrucosa yielded a total of 7.47 g/L (37.4%) and 10.63 g/L (21.26%) of reducing sugars under optimal small (30 mL) and large laboratory-scale (1 L) hydrolysis processes, respectively. Reducing sugar obtained from acid and enzymatic hydrolysates were 10% higher, with minimum by-products, than those reported in other studies. The mass balance for the small laboratory-scale process showed that the acid and enzymatic hydrolysates had a carbohydrate conversion of 57.2%. The mass balance approach to the entire hydrolysis process of red seaweed for biosugar production can be applied to other saccharification processes.

Graphical abstract

High-yield production of biosugars from Gracilaria verrucos by acid and enzymatic hydrolysis processes: a mass balance approach.

Keywords

• Reducing sugars
• Seaweeds
• Chemical hydrolysis
• Enzymatic hydrolysis
• Gracilaria verrucosa

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• DOCX (1.1M)

Supplementary Figs. S1–S3

• ⁎ 
  Corresponding author at: 309, Pilmun-daero, Dong-gu, Gwangju, Republic of Korea. Tel.: +82 62 230 7518, mobile: +82 10 3473 4134; fax: +82 62 230 7226.

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