Analytical method development using FTIR-ATR and FT-Raman spectroscopy to assay fructose, sucrose, glucose and dihydroxyacetone, in Leptospermum scoparium nectar

Published Date
Received 7 September 2015, Revised 22 February 2016, Accepted 27 February 2016, Available online 3 March 2016.

Author
Elizabeth M. Nickless. Author links open the author workspace.a. Numbers and letters correspond to the affiliation list. Click to expose these in author workspaceStephen E. Holroyd. Author links open the author workspace.b. Numbers and letters correspond to the affiliation list. Click to expose these in author workspaceGeorgie Hamilton. Author links open the author workspace.a. Numbers and letters correspond to the affiliation list. Click to expose these in author workspaceKeith C. Gordon. Author links open the author workspace.c. Numbers and letters correspond to the affiliation list. Click to expose these in author workspaceJason J. Wargent. Author links open the author workspace.Opens the author workspaceOpens the author workspacea. Numbers and letters correspond to the affiliation list. Click to expose these in author workspace

a

Institute of Agriculture & Environment, Massey University, Palmerston North, New Zealand

b

Fonterra Co-operative Group, Fonterra Research & Development Centre, Palmerston North, New Zealand

c

Department of Chemistry and Dodd-Walls Centre, University of Otago, Dunedin, New Zealand

Abstract

The carbohydrate dihydroxyacteone (DHA) occurs in significant levels in Leptospermum scoparium (mānuka) nectar and is the precursor of methylglyoxyl (MGO), the unique non-peroxide antibacterial activity (NPA) component in mānuka honey. The nectar of ten different cultivars of L. scoparium was assayed quantitatively for fructose, glucose, sucrose, and DHA with high pressure liquid chromatography (HPLC) for comparison with FT-Raman and FTIR-ATR spectroscopic methods. FT-Raman spectroscopy and ATR-FTIR spectroscopy, alongside chemometric methods using principal component analysis (PCA) and partial least squares (PLS) prediction were shown to be useful techniques to quantify and compare the nectar composition in a range of cultivars of L. scoparium.

Keywords

Dihydroxyacetone

Leptospermum scoparium

FT-Raman

FTIR-ATR

Nectar composition

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