Optimization and microbial community analysis for production of biogas from solid waste residues of palm oil mill industry by solid-state anaerobic digestion

Published Date
Bioresource Technology
August 2016, Vol.214:166–174, doi:10.1016/j.biortech.2016.04.077

• Author 

• Wantanasak Suksong ^a
• Prawit Kongjan ^b
• Poonsuk Prasertsan ^c
• Tsuyoshi Imai ^d
• Sompong O-Thong ^a,e,,

• aBiotechnology Program, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand
• bChemistry Division, Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand
• cDepartment of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand
• dDivision of Environmental Science and Engineering, Graduated school of Science and Engineering, Yamaguchi University, Yamaguchi 755-8611, Japan
• eResearch Center in Energy and Environment, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand

Received 1 February 2016. Revised 15 April 2016. Accepted 16 April 2016. Available online 20 April 2016. 

Highlights

• •
  Solid-state anaerobic digestion (SS-AD) of oil palm biomass was studied.
• •
  Optimal conditions for methane production was 16% TS, C:N of 30:1 and F:I of 2:1.
• •
  The highest methane production of 77.8 m³ CH₄ ton⁻¹ biomass was achieved from EFB.
• •
  Bacteria community in SS-AD was dominated by Ruminococcus sp. and Clostridium sp.
• •
  Archaea community in SS-AD was dominated by Methanoculleus sp.

Abstract

This study investigated the improvement of biogas production from solid-state anaerobic digestion (SS-AD) of oil palm biomass by optimizing of total solids (TS) contents, feedstock to inoculum (F:I) ratios and carbon to nitrogen (C:N) ratios. Highest methane yield from EFB, OPF and OPT of 358, 280 and 324 m³ CH₄ ton⁻¹ VS, respectively, was achieved at TS content of 16%, C:N ratio of 30:1 and F:I ratio of 2:1. The main contribution to methane from biomass was the degradation of cellulose and hemicellulose. The highest methane production of 72 m³ CH₄ ton⁻¹ biomass was achieved from EFB. Bacteria community structure in SS-AD process of oil palm biomass was dominated by Ruminococcus sp. and Clostridium sp., while archaea community was dominated by Methanoculleus sp. Oil palm biomass has great potential for methane production via SS-AD.

Keywords

Solid-state anaerobic digestion

Empty fruit bunches

Oil palm biomass

Biogas production

Microbial community analysis

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• ⁎ 
  Corresponding author at: Biotechnology Program, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand.

© 2016 Elsevier Ltd. All rights reserved.

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