PARENT IDENTIFICATION IN A MULTI LOCATION TRIAL SEED ORCHARD OF ACACIA MANGIUM USING MICROSATELLITE MARKERS

Author

I. L. G. Nurtjahjaningsih

Abstract

Variation of  parent gametes' contribution might affect the growth patterns among offspring produced from seed orchards. This paper studies the mating system statuses and to identify parent trees that produce good growth performance of  offspring in seedling seed orchard of A. mangium. The study was conducted in two seed orchards, i.e. a first generation seedling seed orchard (F1 SSO) of  A. mangium, used as the parent population hereafter and a multi location trial (MLT) as an offspring population. Based on 10 microsatellite markers, mating system in the F1 SSO was under panmictic equilibrium condition. The good growth trees in the MLT originated from various parent genes contribution in the F1 SSO. Several behaviors were observed: a). the best trees in MLT dominantly originated from singlepair trees, between maternal and paternal trees, in F1 SSO; b). a maternal tree was pollinated by two paternal trees; c). a paternal tree pollinated several maternal trees. Flowering synchronization and genetic compatibility among trees might be responsible for these mating system patterns. In order to maximize seeds production, studies on male and female flowers characteristic should be employed to assess flowering synchronization among individual trees in the seed orchard.

Keywords

Parent identification; seedling seed orchard; multi-location trial; microsatellite

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References

Burczyk, J., & Chalupta, W. (1997). Flowering and cone production variability and its effect on parental balance in a Scots pine clonal seed orchard. Ann. For. Sci, 54, 129–144.

Butcher, P. A., Decroocq, S., Gray, Y., & Moran, G. F. (2000). Development, inheritance and cross-species amplification of microsatellite markers from Acacia mangium. Theor Appl Genet, 101, 1282–1290.

Butcher, P., Harwood, C., & Quang, T. H. (2004). Studies of mating systems in seed stands suggest possible causes of variable outcrossing rates in natural populations of Acacia mangium. Forest Genetics, 11(3-4), 303–309.

Chaix, G., Gerber, S., Razafimaharo, V., Vigneron, P., Verhaegen, D., & Hamon, S. (2003). Gene flow estimation with microsatellites in a Malagasi seed orchard of Eucalyptus grandis. Theor Appl Genet, 107, 705–712.

Dering, M., & Chybicki, I. (2012). Assessment of genetic diversity in two-species oak seed stands and their progeny populations. Scandinavian Journal of Forest Research, 27, 2–9.

Dow, B. D., & Ashley, M. V. (1998). High levels of gene flow in bur oak revealed by paternity analysis using microsatellites. Journal of Heredity, 89, 62–70.

El-Kassaby, Y. A., Fashler, A. M. K., & Sziklai, O. (1984). Reproductive phenology and its impact on genetically improve seed production in a Douglass-fir seed orchard. Silvae Genetica, 33(4-5), 120–125.

El-Kassaby, Y. A., Ritland, K., Fashler, A. M. K., & Devitt, W. J. B. (1998). The role of reproductive phenology upon the mating system of a Douglass-fir seed orchard. Silvae Genetica, 37(2), 76–82.

Goudet, J. (2001). FSTAT (version 2.9.3): A program to estimate and test gene diversities and fixatin indices. Retrieved from www.unil. ch/izea/softwares/fsat.html

Hansen, O. K., & Kjaer, E. D. (2006). Paternity analysis with microsatellites in a danis Abies nordmanniana clonal seed orchard reveals dysfunction. Can. J. For. Res., 36, 1054–1058.

Isoda, K., Yuskianti, V., & Rimbawanto, A. (2002). Verification of full sib relationship of seeds within a pod of Acacia mangium using parentage analysis by microsatellite markers. Paper presented at the advance in genetic improvement of tropical tree species in genetic improvement of tropical tree species. Yogyakarta.

Kalinowski, S. T., Taper, M. L., & Marshall, T. C. (2007). Revising how the computer program CERVUS accomodates genotyping error increase success in paternity assignment. Molecular Ecology, 16, 1099–1106.

Kang, K. S. (2000). Clonal and annual variation of flower production and composition of gamete gene pool in a clonal seed orchard of Pinus densiflora. Canadian Journal of Forest Research, 30, 1275–1280.

Larson, E., Hume, G., Andres, J., & Harrison, R. (2012). Post-mating prezygotic barriers to gene exchange between hybridizing field crickets. Journal of Evolutionary Biology, 25, 174–186.

Larson, E. L., White, T. A., Ross, C. L., & Harrison, R. G. (2014). Gene flow and the maintenance of species boundaries. Molecular Ecology, 23, 1668–1678.

Maroja, L., Andres, J., Walters, J., & Harrison, R. (2009). Multiple barriers to gene exchange in a field cricket hybrid zone. Biological Journal of the Linnean Society, 97, 390–402.

Moriguchi, Y., Taira, H., Tani, N., & Tsumura, Y. (2004). Variation of paternal contribution in a seed orchard of Cryptomeria japonica determind using microsatellite markers. Can. J. For. Res., 34, 1683–1690.

Nirsatmanto, A., Leksono, B., Kurinobu, S., & Shiraishi, S. (2004). Realized genetic gain observed in second-generation seedling seed orchards of Acacia mangium in South Kalimantan, Indonesia. Journal of Forest Research, 9, 265–269.

Nurtjahjaningsih, I. L. G., Saito, Y., Tsuda, Y., & Ide, Y. (2007). Genetic diversity of parental and offspring populations in a Pinus merkusii seedling seed orchard detected by microsatellite markers. Bulletin of the Tokyo University Forest, 118, 1–14. Retrieved from http://www.uf.a.u-tokyo.ac.jp/publish/ bulletin/bull/118/01.pdf

Orwa, C., Mutua, A., Kindt, R., Jamnadass, R., & Anthony, S. (2009). Agroforestree database: A tree reference and selection guide version 4.0. Retrieved from http://www. worldagroforestry.org/resources/databases/agroforestree

Robledo-Arnuncio, J. J., & Gil, L. (2005). Patterns of pollen dispersal in a small population of 2526 Pinus sylvestris L. revealed by total-exclusion paternity analysis. Heredity, 94(1), 13–22. doi:10.1038/sj.hdy.6800542

Saro, I., Robledo-Arnuncio, J. J., González-Pérez,M. A, & Sosa, P. A. (2014). Patterns of pollen dispersal in a small population of the Carianan endemic palm (Phoenix canariensis). Heredity, (January), 1–9. doi:10.1038/hdy.2014.16

Shiraishi, S., & Watanabe, A. (1995). Identification of chloroplast genome between Pinus densiflora Sieb et Zucc and P. thumbergii Parl based on the polymorphism in rbcL gene. Journal of Japanese Forestry Society, 77, 429–436.

Thomasset, M., Hodkinson, T. R., Restoux, G., Frascaria-Lacoste, N., Douglas, G. C., & Fernández-Manjarrés, J. F. (2014). Thank you for not flowering: conservation genetics and gene flow analysis of native and non-native populations of Fraxinus (Oleaceae) in Ireland. Heredity, 112(6), 596–606.doi:10.1038/hdy.2013.141

Yuskianti, V., & Isoda, K. (2012). Genetic diversity of Acacia mangium seed orchard in Wonogiri Indonesia using microsatellite markers. HAYATI Journal of Biosciences, 19(3), 141–144. doi:10.4308/hjb.19.3.141

DOI: http://dx.doi.org/10.20886/ijfr.2016.3.1.19-26

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