Author
M.N. Hassan, M.S. Haque, M.M. Hassan and M.S. Haque
Journal of the Bangladesh Agricultural University, 2014, vol. 12
Abstract: Genetic improvement of garlic through conventional breeding is very difficult due to sterile nature of its flower. Hence, an alternative system is desirable to induce genetic variation. Tissue culture could be a good opportunities and somatic embryogenesis is one of the potential techniques of tissue culture for in vitro regeneration of garlic plant. The successes and production of somatic embryo depends on several factors such as optimization of media components, genotypes and explant type. Therefore, in the present investigation, garlic root tips were used as explant for callus and somatic embryo induction under different plant growth regulator combination. It was found that MS+1.0 mg l-1 2,4-D was the most favorable (86.10% regeneration with 2.19 cm callus diameter) for callus induction. This concentration also induced and produced good quality somatic embryo. In addition, MS+2.0 mg l-1 Kinetin gave better regeneration of somatic embryo and yielded the highest number (4.670) and longest length (7.0 cm) of shoots per callus. The procedure used a single hormonal signal for callus and somatic embryo induction as well as hormone free medium for further development of plantlet. Besides, maximum duration for callus induction and somatic embryo production was 17 and 10.67 days respectively. Thus, it appears that the protocol is cheap and time bound and particularly useful for conducting experiment for genetic improvement of garlic. Furthermore, as the protocol is cost effective, it can be further tested for commercial feasibility.
Keywords: Root tip; Explant; In vitro culture; Somatic embryogenesis; Plantlet regeneration; Crop Production/Industries (search for similar items in EconPapers)
Date: 2014
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http://econpapers.repec.org/article/agsbdbjaf/209886.htm
M.N. Hassan, M.S. Haque, M.M. Hassan and M.S. Haque
Journal of the Bangladesh Agricultural University, 2014, vol. 12
Abstract: Genetic improvement of garlic through conventional breeding is very difficult due to sterile nature of its flower. Hence, an alternative system is desirable to induce genetic variation. Tissue culture could be a good opportunities and somatic embryogenesis is one of the potential techniques of tissue culture for in vitro regeneration of garlic plant. The successes and production of somatic embryo depends on several factors such as optimization of media components, genotypes and explant type. Therefore, in the present investigation, garlic root tips were used as explant for callus and somatic embryo induction under different plant growth regulator combination. It was found that MS+1.0 mg l-1 2,4-D was the most favorable (86.10% regeneration with 2.19 cm callus diameter) for callus induction. This concentration also induced and produced good quality somatic embryo. In addition, MS+2.0 mg l-1 Kinetin gave better regeneration of somatic embryo and yielded the highest number (4.670) and longest length (7.0 cm) of shoots per callus. The procedure used a single hormonal signal for callus and somatic embryo induction as well as hormone free medium for further development of plantlet. Besides, maximum duration for callus induction and somatic embryo production was 17 and 10.67 days respectively. Thus, it appears that the protocol is cheap and time bound and particularly useful for conducting experiment for genetic improvement of garlic. Furthermore, as the protocol is cost effective, it can be further tested for commercial feasibility.
Keywords: Root tip; Explant; In vitro culture; Somatic embryogenesis; Plantlet regeneration; Crop Production/Industries (search for similar items in EconPapers)
Date: 2014
References: View complete reference list from CitEc
Citations Track citations by RSS feed
Downloads: (external link)
http://purl.umn.edu/209886 (application/pdf)
Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.
Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text
Persistent link: http://EconPapers.repec.org/RePEc:ags:bdbjaf:209886
Access Statistics for this article
More articles in Journal of the Bangladesh Agricultural University from Bangladesh Agricultural University Research System (BAURES) Contact information at EDIRC.
Series data maintained by AgEcon Search (aesearch@umn.edu).
For further detail slog on website :
http://econpapers.repec.org/article/agsbdbjaf/209886.htm
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