Efficient enzymatic degradation process for hydrolysis activity of the Carrageenan from red algae in marine biomass

Published Date
Journal of Biotechnology
20 December 2014, Vol.192:108–113, doi:10.1016/j.jbiotec.2014.09.019

• Author 

• Dae Hee Kang ^a,1
• Jeong Eun Hyeon ^a,1
• Seung Kyou You ^a
• Seung Wook Kim ^b
• Sung Ok Han ^a,,,

• aDepartment of Biotechnology, Korea University, Seoul 136-701, Republic of Korea
• bDepartment of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea

Received 6 June 2014. Revised 22 September 2014. Accepted 24 September 2014. Available online 2 October 2014. 

Highlights

• •
  Effectively overcomes the limitations associated with pretreatment of substrate.
• •
  Hydrolysis product such as oligosaccharides to economically produce.
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  Created a new protein complex at the type of κ- and λ-carrageenase enzyme.
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  Relatively low-cost, high-efficiency purification effect using CBM purification process.
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  Demonstrates that diversity of marine biomass research with availability of industrial.

Abstract

Carrageenan is a generic name for a family of polysaccharides obtained from certain species of red algae. New methods to produce useful cost-efficiently materials from red algae are needed to convert enzymatic processes into fermentable sugars. In this study, we constructed chimeric genes cCgkA and cCglA containing the catalytic domain of κ-carrageenase CgkA and λ-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain. Recombinant strains expressing the chimeric carrageenase resulted in a halo formation on the carrageenan plate by alcian blue staining. The recombinant cCgkA and cCglA were assembled with scaffoldin miniCbpA via cohesin and dockerin interaction. Carbohydrate binding module (CBM) in scaffoldin was used as a tag for cellulose affinity purification using cellulose as a support. The hydrolysis process was monitored by the amount of reducing sugar released from carrageenan. Interestingly, these results indicated that miniCbpA, cCgkA and cCglA assembled into a complex and that the dockerin-fused enzymes on the scaffoldin had synergistic activity in the degradation of carrageenan. The observed enhancement of activity by carrageenolytic complex was 3.1-fold-higher compared with the corresponding enzymes alone. Thus, the assemblies of advancement of active enzyme complexes will facilitate the commercial production of useful products from red algae biomass which represents inexpensive and sustainable feed-stocks.

Keywords

Carrageenase

Scaffoldin

Cohesin

Dockerin

Carbohydrate binding module

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• ⁎ 
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Copyright © 2014 Elsevier B.V. All rights reserved.

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